Journal: Journal of Clinical Pathology

Article Title: Unusual fusion gene rearrangements in patients with nodular fasciitis: a study of rare and novel USP6 fusion partners with a review of the literature

doi: 10.1136/jcp-2023-208768

Figure Lengend Snippet: Patient characteristics and clinical findings in USP6::MYH9 fusion cases

Article Snippet: Thin histological sections (3 μm thick) were used, and each sample was stained using the following antibodies and protocols: anti-smooth muscle actin (SMA) mouse monoclonal antibodies (clone 1A4, BioSB— Bioscience for the World, dilution 1:75; pretreatment: heating up to 99°C in a pH 6 buffer in a water bath); anti-H-caldesmon mouse monoclonal antibodies (clone BSB-19|, BioSB, dilution 1:100; pretreatment: heating up to 99°C in a pH 9 buffer in a water bath); anti-desmin mouse monoclonal antibodies (clone D33, BioSB, dilution 1:100; pretreatment: heating up to 99°C in a pH 9 buffer in a water bath); anti-S100-β rabbit monoclonal antibodies (clone EP32, BioSB, dilution 1:300; pre-treatment: heating up to 99 ◦C in a pH 9 buffer in a water bath); anti-Ki-67 mouse monoclonal antibodies (clone MIB-1, BioSB, dilution 1:150; pretreatment: heating up to 99°C in a pH 6 buffer in a water bath).

Techniques:

Journal: Cell Reports

Article Title: Multi-site investigation of gut microbiota in CDKL5 deficiency disorder mouse models: Targeting dysbiosis to improve neurological outcomes

doi: 10.1016/j.celrep.2025.115546

Figure Lengend Snippet:

Article Snippet: Cortical sections were incubated for 2 h in a blocking solution containing 10% BSA (w/vol) and 0.3% Triton X-100 (vol/vol) in PBS and incubated overnight at 4°C with rabbit polyclonal anti-S100β primary antibody (GeneTex, Cat. no. GTX129573; RRID: AB_2886037 ) diluted 1:250, in PBS with 5% BSA (w/vol) and 0.15% Triton X-100 (vol/vol).

Techniques: Recombinant, SYBR Green Assay, Plasmid Preparation, Reverse Transcription, Mutagenesis, Software, Imaging, Spectrophotometry, Real-time Polymerase Chain Reaction, Microscopy